The Pipsyl Method

The Pipsyl Method. Using radioactive isotopes Keston, Udenfriend and Cannan (1946, 1949 Keston and Udenfriend, 1949) have developed an accurate micro method, which theoretically could be applied to the estimation of any amino acid or peptide in a mixture. Two different techniques, the carrier technique and the indicator technique, have been worked out. In the former the peptide mixture to be analyzed is treated with p-iodophenylsulfonyl chloride (pipsyl chloride) containing radioactive which reacts quantitatively with the amino groups giving pipsyl 

In this section we shall consider the results obtained since 1943, which provide information concerning the arrangement of amino acids in proteins. Similar studies on the naturally occurring polypeptides will not be dealt with here. 

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