The Choice of Primary Antibodies

If possible, your primary antibody should not be raised in the same species as the tissue under study, since antigen detection with the use of antibodies on 

Source Type Quantity of total antibody Quantity of specific antibody Contaminating antibodies Purity of specific antibody 

Serum Polyclonal 10 mg/ml 1 mg/ml Other serum antibodies 10% (except for antigen affinity column) 

Culture supernatants with 10% FCS Monoclonal 1 mg/ml 0.01 0.05 mg/ml Background from calf serum 95%, no cross reaction, high quality 

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