Tetracycline Rolitetracycline

Immunochemical methods have been developed and placed on the market to analyze tetracycline residues (see Table 4). Thus, a qualitative EIA has been developed and used to analyze tetracyclines in honey samples with a detection level of 20 pg/kg-1 [96]. A microplate-based indirect ELISA has been developed to analyze tetracyclines using polyclonal antibodies. The assay could measure tetracycline in the range between 0.1 and 6 ng mL L Other tetracycline antibiotics such as chlortetracycline, rolitetracycline, or minocycline are also highly recognized in this assay [98]. Several immunoassay kits are commercially available for the analysis of tetracyclines although, to our knowledge, none of them... 

Rolitetracycline (— yellow-brown), tetracycline (— yellow-brown)... 

Bendrofluazide, chlorotrianisene, chromonar, clofazimine, cyproheptadine, dothiepin, mesoridazine (— blue), methylenedioxyamphetamine, nicergoline, perazine, phenindione, rolitetracycline, tetracycline... 

Rolitetracycline, the Mannich base derived from the carboxamide function of tetracycline, formaldehyde and pyrrolidine, is surprisingly stable and is used as an injectable form of tetracycline. 

A. G. Butterfield, D. W. Hughes, W. L. Wilson, and N. J. Pound, Simultaneous high-speed liquid chromatographic determination of tetracycline and rolitetracycline in rolitetracycline formulations, J. Pharm. Sci. 64, 316-320 (1975). 

Tetracyclines Chlortetracyline, Demeclocycline, Doxycycline, Lymecycline, Methacycline, Minocycline, Oxytetracycline, Rolitetracycline, letracycline, Tigecycline... 

G. Goissis, and M.H. De Sousa, Characterization and in vitro release studies of tetracycline and rolitetracycline imobilized on anionic collagen membranes. Materials Research, 12(1), 69-74, 2009. 

The same group of authors published a paper (52) summarizing established optimal conditions for the analysis of tetracyclines by means of HPLC and HPTLC including methacycline (MC), demeclocycline (DMC), rolitetracycline (RTC), and minocycline (MI). The chromatographic systems for the separations have already been described (determination of tetracyclines in honey, silica gel RP-8) (50) scanning was performed at 350 nm (see Fig. 5 and Table 23). 

Rolitetracycline is more stable in tetrahydrofuran and dimethylsulfoxide than in methanol, acetonitrile, and dioxane. It was proposed to convert rolitetracycline into tetracycline by heating the methanolic solution for 5 min at 50 C, an to determine tetracycline in order to avoid problems due to the instability or rolitetracycline. 

Figure 5 Separation of tetracyclines on silicagel HPTLC (A) and RP 8 (B) plates (both Merck). A pre-devel-oping with saturated Na2EDTA-solution and activating for 2 h at I30 C Solvent chloroform-methanol-S% aqueous Naa EDTA (65 20 5, lower layer). B Solvent methanol-acetonitrile-0,5 M oxalic acid (1 1 4, pH = 3.0) 0.1-1.0 pg/spot. Abbreviations MI = minocycline, CTC = chlortetracycline, DMC = demeclocycline, TC = tetracycline, DC = doxycycline, MC = methacycline, OTC = oxytetracycline, RTC = rolitetracycline (unstable).

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