Testing before using an additive

Before any additive is to be used with a given cell type, it should first be tested to ensure that it has no detrimental effects on cell growth, metabolism, differentiation, protein expression, etc., and that it does indeed offer mechanical protection in agitated and/or aerated systems (bioreactors). 

Run cultures in well plates or T-flasks (in duplicate or triplicate) with and without the additive. 

Use an average, and the highest reported concentration (see below), of the additive for the initial screening. If a new additive is being tested, use a range of concentrations. 

If a small inhibitory effect is found (which is quite usual), proceed to assess whether or not the additive offers the desirable mechanical protection. 

If the additive offers mechanical protection, check if the protection effect is present at lower non-inhibitory concentrations. Most cells can overcome a small inhibitory effect by being acclimatized to the additive. 

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