Tertiary structure determination

Most proteins contain more than one polypeptide chain. The manner in which these chains associate determines quaternary structure. Binding involves the same types of noncovalent forces mentioned for tertiary structure van der Waals forces, hydrophobic and hydrophilic attractions, and hydrogen bonding. However, the interactions are now interchain rather than infrachain (tertiary structure determination). The quaternary structure of hemoglobin (four almost identical subunits) will be discussed in Chapter 4, that of superoxide dismutase (two identical subunits) will be discussed in Chapter 5, and that of nitrogenase (multiple dissimilar subunits) will be discussed in Chapter 6. 

Godzik, A., and Skolnick, J. (1992). Sequence-structure matching in globular proteins application to supersecondary and tertiary structure determination. Proc. Natl. Acad. Sci. U.S.A. 89, 12098-12102. 

Dinitrogenase has been crystallized and its tertiary structure determined by Kim and Rees. [44, 45,46] As indicated, an Fe-Mo unit serves at the active site. Electrons are furnished to this active unit by the Fe enzyme dinitrogenase reductase. The two units together constitute nitrogenase. 

It has been shown in several biological assembly systems that, due to the fact that tertiary structure determines quaternary structure, a major component of the system can be assembled on its own to form structures similar to the intact organelle (Caspar and Klug, 1962). Ex-... 

Restrained Molecular Dynamics Procedure for Protein Tertiary Structure Determination from NMR Data A lac Repressor Headpiece Structure Based on Information on /-Coupling and from Presence and Absence of NOEs. 

Myoglobin, the first protein to have its tertiary structure determined, is a globular protein for oxygen storage. 

J. De Vlieg, R. Boelens, R. M. Scheek, R. Kaptein, and W. F. van Gunsteren, hr. J. Chem., 27, 181 (1986). Restrained Molecular Dynamics Procedure for Protein Tertiary Structure Determination from NMR Data A lac Repressor Headpiece Structure Based on Information on J-Coupling and from Presence and Absence of NOE s. 

Gomis-Ruth, F-X., Kress, L.F., Kellerman, J., Mayr, I., Lee, X., Huber, R. and Bode, W., 1994, Refined 2.0 A x-ray crystal structure of the snake venom zinc-endopeptidase adamalysin II. Primary and tertiary structure determination, refinement, molecular structure and comparison with astacin, collagenase and thermolysin, J. Mol. Biol. 239 513-544. 

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