Template 10 region

Yokoyama Y, Takahashi Y, Shinohara A, Lian Z, Wan X, et al. 1998. Attenuation of telomerase activity by a hammerhead ribozyme targeting the template region of telomerase RNA in endometrial carcinoma cells. Cancer Res. 58 5406-10... 

Messenger RNA, synthesized in the living cell by the action of an enzyme that carries out the polymerization of ribonucleotides on a DNA template region that carries the information for the primary sequence of amino acids in a structural protein. It is a ribonucleotide copy of the deoxynucleotide sequences in the primary genetic material. 

Fig. 1 Activity of telomerase and its inhibition by GRN163L. Telomerase is a reverse transcriptase that uses an internal RNA template (10 nucleotides white letters over dark gray box) to synthesize dotted arrow RT) a 6-base telomeric DNA repeat. GRN163L hybridizes to the template region of telomerase and to surrounding nucleotides to block the activity of the enzyme...

A sequence alignment establishes the correspondences between the amino adds in th unknown protein and the template protein (or proteins) from wliich it will be built. Th three-dimensional structures of two or more related proteins are conveniently divided int structurally conserved regions (SCRs) and structurally variable regions (SVRs). Ihe structural conserved regions correspond to those stretches of maximum sequence identity or sequenc... 

Backbone generation is the first step in building a three-dimensional model of the protein. First, it is necessary to find structurally conserved regions (SCR) in the backbone. Next, place them in space with an orientation and conformation best matching those of the template. Single amino acid exchanges are assumed not to affect the tertiary structure. This often results in having sections of the model compound that are unconnected. 

FIGURE 28 10 During transcription a molecule of mRNA is assembled from a DNA template Transcription begins at a promoter sequence and proceeds in the 5 3 direction of the mRNA until a termination sequence of the DNA is reached Only a region of about 10 base pairs is unwound at any time... 

The core of the network is the region of the rail network in which the branch line of two transformer substations or of any other branch line is less than 2 km distant in a direct line. All branch lines outside the core of the network are termed outlying lines. In a branched rail network, not only the stretches of line within a circle with a radius of 2 km from the most negative return current point of a transformer substation belong to the core of the network, but also connecting branch lines that are less than 2 km from each other [1]. The area of a network core can be simply determined on a track plan with the help of a circular template as in Fig. 15-1. 

Distortions or shifts of a region that is aligned correctly with the template structures. 

In order to examine whether this sequence gave a fold similar to the template, the corresponding peptide was synthesized and its structure experimentally determined by NMR methods. The result is shown in Figure 17.15 and compared to the design target whose main chain conformation is identical to that of the Zif 268 template. The folds are remarkably similar even though there are some differences in the loop region between the two p strands. The core of the molecule, which comprises seven hydrophobic side chains, is well-ordered whereas the termini are disordered. The root mean square deviation of the main chain atoms are 2.0 A for residues 3 to 26 and 1.0 A for residues 8 to 26. 

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