Techniques of the Zimm Plot

The radius of gyration, 5, gives an indication as to whether a chain is compact or extended. If additional informatgion, such as shape, is available, we may estimate the size  

First a stock solution is prepared, for example, 5.2153 x 10 g/mL, and, labeled as 1 unit. The dilution is labeled as, and units. Thus, the concentrations are always 1, 2, 3, and 4 (unit stock concentration, e.g., (5.2153 x 10 g/mL) or j, 1 (unit stock concentration). 

Light scattering is then measured /, the intensity of scattered light Note that 

The An/Ac values are obtained from the measurement of the solutions (same solutions as for light-scattering apparatus) with a differential refractometer (a separate instrument). 

Arbitrarily select 0, 1 units for the abscissa For the c = line, we line up the 

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