TCA cyde intermediates

The answer is that not only is there no apparent regeneration of oxaloacetate there is seemingly no way of producing any of the TCA cyde intermediates from sucdnyl CoA through to oxaloacetate. 

Give three reasons why it is believed that the formation of itaconic add in A. terreus is via a route which does not involve TCA cyde intermediates. 

Exopolysaccharide production ntay be improved by tiie provision of various organic con xments, otiier Aan the main carbon and energy source. These can improve growth of the production organism (growth factors) and/or directly enhance the synthesis of exopolysaccharide. Additions that improve polymer yield include tricarboxylic add (TCA) cyde intermediates, which are thou t to improve metabolic balance between carbon flow frcnn carbohydrate substrate through the catabolic pathwaj arul oxidation through the TCA cyde. 

In addition to dtrate, there are other addic intermediates of file TCA cyde and several of these are used in industry. Even though some have been produced by fermentation, production is currently from other, cheaper sources. 

The nitrogen source in the medium is the amino add glutamate. There are several cations K Mn2, Cn2, Zn2, Mg2, Co2, Fe2, Ca2 Mo6. Phosphate (POi") is the major anionic component. Fumaric add is a TCA cycle intermediate and may improve metabolic balance through the catabolic pathways and oxidation through the TCA cyde. Peptone may improve growth through the provision of growth factors (amino acids, vitamins, nudeotides). 

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