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System for Integrative Genomic

Michielse CB, Salim K, Ragas O, Ram AFJ, Kudla B, Jarry B, Punt PJ, van den Hondel CAMJJ. (2004). Development of a system for integrative and stable transformation of the Zygomycete Rhizopus oryzae by Agrobacterium-mediated DNA transfer. Mol Genet Genomics, 271,499-510. [Pg.432]

Applying mass-balance and thermodynamic constraints typically leaves one without a precisely defined (unique) solution for reaction fluxes and reactant concentration, but instead with a mathematically constrained feasible space for these variables. Exploration of this feasible space is the purview of constraint-based analysis. It has so far been left unstated that any application in this area starts with the determination of the reactions in a system, from which the stoichiometric matrix arises. This first step, network reconstruction, integrates genomic and proteomic data to determine carefully the enzymes present in an organism, cell, or subcellular compartment. The network reconstruction process is described elsewhere [107]. [Pg.238]

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