Syringe loading suspension

Syringe Loading A Method for Inserting Macromolecules into Cells in Suspension... 

Load the liposome suspension into one of the syringes (donor syringe) of the mini extruder and carefully place the syringe into one end of the extruder by applying a gentle twisting. 

Suspensions of each of the four tracers were prepared. Biological agents were diluted approximately 1 20 from the stock suspensions and vortex mixed. Once the syringes were loaded with tracer and the electrodes were inserted, the difference in hydrostatic pressure between syringes was balanced by adjusting the level of background solution in the downstream syringe until flow was observed to stop. 

Reaction Procedure (Scheme 2.17) A Schlenk tube equipped with a strong magnetic stir bar was charged with Cul (57 mg, 0.3 mmol, 0.15 equiv.), morpholine-4-carboxamidine hydrobromide (588 mg, 2.8 mmol, 1.4 equiv.), and CS2CO3 (2.6 g, 8 mmol, 4 equiv.). The Schlenk tube was evacuated and backfilled with N2 three times. Under a N2 atmosphere, DMA or NMP (5 mL), 1,2-diiodobenzene (659 mg, 2 mmol, 1.0 equiv.) and iV,iV -dimethylethylenediamine (53 mg, 0.6 mmol, 0.3 equiv.) were added sequentially via syringe. The reaction mixture was stirred in a preheated oil bath at 150 °C for 24 h and then cooled to room temperature. EtOAc (25 mL) was added, and the suspension was stirred for 30 min. The inorganic salt was filtered off and washed with EtOAc. The volatile EtOAc was removed under reduced pressure, and the remaining DMA or NMP solution was directly loaded on a preparatory HPLC for purification to afford the product. 

Figure 38. A Lovrien three-channel batch heat conduction microcalorimeter for solution-solution or solution-suspension mixing. Each vessel (5 cm total) has two compartments. The smaller chamber (shown being loaded with the syringe) holds 0.5-1.0-cm while the larger chambers can receive 1.0-2.0 cm. After thermal equilibration, 6 to 15 min, the assembly is inverted to mix all the vessels contents and thus start the reactions. Two Seebeck thermopiles make contact with the sides of each vessel. The reference vessel s thermopiles are connected in opposition to the thermopiles of all the three sample vessels. Thus, the heat of the reference or control experiment is electrically subtracted from the heat of each of the three sample vessels. Port closures may be fitted for the gas purging necessary for either aerobic or anaerobic conditions. The mixing vessel materials are glass, 18 K gold or stainless steel (Reproduced from Reference [131] with permission).

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