Synthetic affinity ligand

Direct physicochemical analysis of synthetic affinity ligands is difficult when immobilized to the solid-phase bead (i.e., in situ). Solution-phase synthesis of lead compounds is often performed to allow characterization of the ligand by H-NMR, C-NMR, and mass spectrometry (MS) techniques (6, 10, 12, 14). We describe, herein, the synthesis of the solution-phase modified Ugi ligand, PpL-mimic (amine l-amino-2-naphthol carboxylic acid pentanoic acid isonitrile isopropyl isocyanide aldehyde acetaldehyde) (see Note 13). 

A very similar cloning strategy has been used for the 48-kDa subunit of the glycine-receptor channel by Betz and collaborators [4]. Strychnine was used as an affinity ligand, and synthetic oligopeptides were constructed from the purified material. They have been used to screen cDNA libraries. 

Model system for testing utilization of immobilized affinity peptides - SMC (CIM ) optimally present small affinity ligands Ligand utilization, comparison with agarose, cellulose and synthetic particle based polymers Affinity (peptides) disks [85]... 

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