Synthesis using glycosyltransferases

Highly selective enzymatic synthesis using glycosyltransferases (GTFs), an approach restricted by the limited availability of Leloir-type enzymes, and expensive nucleotide-activated substrates... 

The general scheme for solid-phase synthesis using glycosyltransferases is depicted in Scheme 1. Solid-phase synthesis requires a covalent linker group to attach the small molecule, a monosaccharide or an amino acid in the case of a glycopep-tide, on to the polymeric resin. Such a linker must be stable to the reaction con-... 

Cyclodextrins (CDs) have the ability to include the hydrophobic area of a compound, and the formed complex becomes soluble in water due to hydroxyl groups orientated on the surface of CDs. Thus, it was proposed that CDs would have similar properties as surfactants in enzymatic glycosylations. Nishimura etuL studied the utility of simple CDs for the synthesis of neoglycolipids using glycosyltransferase. In this approach, the authors attempted to use cell-media synthesis, which was named cellular-chemoenzymatic synthesis, as a triple-factor combination method, to construct oligosaccharide easily. Three types of CD, a-CD, /3-CD, and... 

Scheme 1. General scheme for the solid-phase synthesis of oligosaccharides using glycosyltransferases.

Abe, H., Shimma, T. I. Jigami, Y. (2003). In vitro oligosaccharide synthesis using intact yeast cells that display glycosyltransferases at the cell surface through cell wall-anchored... 

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