Synthesis Strategies with Transaminases

Consequently, it is important to gain further knowledge about the structure and the mechanism of the relevant enzymes to facilitate in protein engineering in order to expand the substrate and thus the product range, leading to the desired optically pure enantiomers. 

General synthesis strategies for transaminase-catalyzed reactions. (a) Asymmetric synthesis with transaminase, (b) Kinetic resolution with transaminase, (c) Dynamic kinetic resolution with transaminase, (d) One-pot two-step deracemization with transaminase. 

Product Enzyme Substrate Synthesis Strategy Reference 

Pyrrolidine (R)/(S)-(b-TA Vibriofluvialis, Chromobacterium violaceum, ATA-117) (rflc)-4-Oxo-3-phenylbutyric acid+l/ o-alanine DKR [37] 

Rivastigmine co-TA Paracoccus denitrificans) 3-Acetylphenyl ethyl(methyl)carbamate+L-alanine AS [39] 

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