Synthesis and Estimation of Canolol

Various Analytical Methods for Sinapine and Related Compounds 

Reversed-jiiase high-performance Uquid chromatography (RP-HPLC) to detect sinapine from selected cruciferous plants AlkaU treatment of sinapine to choUne and sinapic acid followed by enzymatic hydrolysis of choline to betaine by choline oxidase 

Ion-exchange column purification procedure using CM-Sephadex C25 to estimate sinapine content in the seed or meal 

Used for separating 1-O-glycopyranosyl sinapate from rapeseed liquid-assisted surface desorption atmosphmc pressure chemical ionization mass spectrometry (DAPCI-MS) to detect sinapine in radish taproot tissue Sinapine formed a coloured complex with titanium tetrachloride Based on the yellow colour of sinapine in alkaUne media and the weakening of the intensity of the red coloured Fe-sulphosalicylic acid complex in the presence of phytic acid. 

Enzymatic reduction of sinapic acid ester content in canola meal using polyphenol oxidase from the fungus T. versicolor 

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