Synchronous culture operation

Type Method System Growth rate Technique 

Asynchronous Batch Closed Changing Traditional Bactogen  

Synchrony Batch Closed Changing Synchronous Synchronous 

Two general classes of methods exist for attaining synchrony in batch and/or continuous culture  

Methods whereby cells at the same stage of division are selected or separated from a randomly dividing population (usually by a mechanical or physical procedure) 

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The influence of the OTR on kinetics (and therefore on productivity) was indicated in Equs. 5.169 and 5.170. Therefore, we will discuss here primarily those process engineering factors involved in various different types of reactors and operations. Last but not least, some unconventional reactors such as membrane (dialysis) reactors and synchronous culture techniques will be discussed. 

It is often difficult to avoid introducing some degree of synchronous growth in a culture simply as a results of routine operations. Thus Stubblefield et al. (1967) found that subculture of Don-C cells every 24 h exerted a selection pressure favouring cells with a 12 h generation time and highest mitotic frequencies at about 7 and 19 h. 

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