Surfactant carbohydrate determination

Suppliers of visible spectrophotometers are reviewed in Table 1.1. Spectroscopic methods are applicable to the determination of phenols, chlorophenols, amines, mixtures of organics, boron, halogens, total nitrogen and total phosphorus in soils, cationic surfactants, carbohydrates, total nitrogen, phosphorus and sulphur in non-saline sediments, boron, total organic carbon, total sulphur and arsenic in saline sediments, cationic surfactants, adenosine triphosphate and total organic carbon in sludges. 

Thereafter, approximate quantitative determinations of the carbohydrate content of the microbubble glycopeptide surfactant were made through the use of degradative enzymes. Table 4.3 summarizes the results from polyacrylamide (slab) gel electrophoresis of glycopeptide surfactant treated with P-N-acetyl-hexosaminidase, both alone and with endoglycosidase H. The... 

The evaporative light-scattering detector (ELSD) is a near universal detector suitable for the determination of (mainly) neutral compounds that are less volatile than the mobile phase used for the separation [151,152]. Primary uses include the detection of compounds with a weak response to the UV detector, especially carbohydrates, lipids, surfactants, polymers and petroleum products. Its greater sensitivity and ease of use in gradient elution separations makes it preferable to the refractive index detector for these applications. The ELSD is compatible with most volatile solvents used for normal and... 

The examples shown illustrate only a few of the possible NMR applications. There are other qualitative and quantitative NMR applications, for example the determination of citric acid, acetic acid, acetone, ethanol, methanol, carbohydrates, ceramides and plasmalogens in lecithin polyethyleneglycol-based emulsifiers in animal feed (limit 100 ppm) emulsifiers, surfactants and preservatives in cosmetics (Spectral Service, unpublished). 

The molecular weights of the receptor protein moieties were also determined as above, but using 0.1 % sodium deoxycholate (NaDOC) as a surfactant Instead of SDS. The values obtained were 430,000 for the dimer and 220,000 for the monomer. Taking into account Che carbohydrate content (about 6 %) of Narke receptor proteins, the molecular weights were estimated to 460,000 for the dimer and 240,000 for the monomer. 

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