Surface Plasmon Resonance Analysis

Center for Fluorescence Spectroscopy, University of Maryland, School of Medicine, 725 W. Lombard Street, Baltimore, MD 21201 

Topics in Fluorescence Spectroscopy, Volume 8 Radiative Decay Engineering 

Edited by Geddes and Lakowicz, Springer Science-t-Business Media, Inc., New York, 2005 

The theory of SPR involves the solution of the Maxwell equation with proper boundary conditions [23-25]. The optical and reflective properties of silver and gold depend on the interplay of incident frequency and electron mobility, as well as underlying absorption bands not related to electron oscillations. In this chapter, we will present only an intuitive approach to the SPR phenomenon. For a more detailed description, we refer readers to [19-21,26]. 

For SPR measurement the incident beam should be p-polarized (the electric vector is parallel to the plane of incidence) since s-polarized light will not excite the surface plasmon and will not decrease reflectivity at some angles of incidence. Similarly to a total internal reflection (TIR), the incident beam which excites surface plasmons creates an evanescent field. This evanescent field penetrates the medium next to the metal up to a few hundred nanometers [27-29] and can provide effective excitation of fluorophores. 

---

Rasooly A., Surface plasmon resonance analysis of staphylococcal enterotoxin B in food, Journal of Food Protection 2001 64 37-43. 

Green RJ, Frazier RA, Shakesheff KM, Davies MC, Roberts CJ, Tendler SJB (2000) Surface plasmon resonance analysis of dynamic biological interactions with biomaterials. Biomaterials 21 1823-1835... 

Phillips KS, Han J-H, Martinez M, Wang Z, Carter D, Cheng Q (2006) Nanoscale classifcia-tion of gold substrates for surface plasmon resonance analysis of protein toxins with supported lipid membranes. Anal Chem 78 596-603... 

Figure 10 Surface plasmon resonance analysis. A surface displaying ligand is examined under conditions of flow. Injection of lectin results in an increase in signal observed in the association phase of the curve. At equilibrium the surface achieves maximal binding. Measurement of the dissociation can yield off-rates. Competing ligands in solution can also be assayed.

Surface Plasmon Resonance Sensors review published in Sensors and Actuators B 54 3-15 (1999) and review of surface plasmon resonance analysis of dynamic biological interactions with biomolecules in Biomaterials 21 1823-1835 (2000). 

Brinen, J.S., Rosati, I,., Chakel, J. and Lindley, P. (1993) The effect of polymer architecture on the SIMS spectra of glvcolide / trimethylene carbonate copolymers. Surface and Interface. Analysis, 20, 105.5-1060. Burnham,. kA. and Colton, R.J. (1993) Scanning Tunneling Microscopy and Specuoscopy Theory, Techniques and Applications. Force microscopy, ed. Bonnell D.A., New York, VCl I Publishers Inc. Chen, X., Shakesheff, KM., Davies, M.C., Heller, J., Roberts, C.J., Tendler, S.J.B, and Williams, P.M. (1995) The degradation of a thin polymer film studied by simultaneous in situ atomic force microscopy and surface plasmon resonance analysis. Journal of Physical Chemistry, 11, 2547. 

ShakeshefE K.M., Chen, X., Datles, M.C., Domb, A., Roberts, C.J., Tendler. SJ.B. and Williams, P.M. (1995) Relating the phase morphology of a biodegradable polyaner blend to ero.sion kinetics using simultaneous in situ atomic force microscopy and surface plasmon resonance analysis., Langmuir, 11, 3921-3927. 

Figure 1. Typical configuration for surface-plasmon resonance analysis. The incident beam is p-polarized (adopted...

---