SUPREX studies

We acknowledge the assistance of Nikhil S. Dodhiwala who performed most of the SFE work, of Janet Benedicto and Lisa Balch, who performed all gas chromatographic and gas chromatographic/mass spectrometric analyses reported in this manuscript, and of Karin Bauer of Midwest Research Institute, who provided input on the statistical analysis of the preliminary results from our method optimization study. We would also like to acknowledge the assistance of Ashok Shah and Carl Stadler of Suprex Corporation who helped with the design of the dual-extraction setup on the Suprex SE-50 system, and of Hewlett-Packard Company who made available to us a Hewlett Packard extractor. 

In the second part of the chapter, the application of MALDI mass spectrometry for probing noncovalent interactions will be discussed. While special precautions have to be taken to avoid dissociation of complexes during MALDI sample preparation and during the desorption/ionization step, there are some extremely useful features of MALDI that make it suitable for fast readout in solvent accessibility studies (via HDX) and even for the determination of noncovalent binding constants via the so-called SUPREX method. 

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