Supercritical fluid chromatography using chiral stationary phases

4 Supercritical fluid chromatography using chiral stationary phases 

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Svensson, L.A. and Owens, P.K., Enantioselective supercritical fluid chromatography using ristocetin A chiral stationary phases. Analyst, 125, 1037, 2000. 

Kaida, Y. and Okamoto, Y. (1992) Optical resolution by supercritical fluid chromatography using polysaccharide derivatives as chiral stationary phases, Bull. Chem. Soc. Jpn. 65, 2286-2288. 

K.L. Williams, L.C. Sander and S.A. Wise, Comparison of Liquid and Supercritical Fluid Chromatography Using Naphthylethylcarbamoylated- 3-cyclodextrin Chiral Stationary Phases, J. Chromatogr. A, 746(1996)91. 

A polar stationary phases such as ODS and TMS and polar such as CN, diol, or NO2 have been successfully applied to the separation of monosaccharides Separation of maltodextrin derivatized with trifluoroacetic anhydride using FID and a CN packed column Capillary supercritical fluid chromatography-MS is well suited to confirm the identity of the TMS derivative of inositol triphosphate Chiral analytes Enantioseparation... 

Chiral Stationary Phases for GC and HPLC. Enantiomerically pure NEA has been used to prepare a variety of chiral stationary phases for liquid, gas, and supercritical fluid chromatography. These stationary phases are used to separate enantiomers without derivatization of the substrate with a chiral agent. 

There are two general approaches for the separation of enantiomers [1-4,28-32]. The direct method is based on the formation of transient diastereomer association complexes with a chiral selector immobilized in the stationary phase, or added to the mobile phase. The former approach requires the use of special stationary phases (section 10.4) while the later uses conventional stationary phases with special additives included in the mobile phase (section 10.5). When preparative applications are contemplated the use of immobilized chiral selectors is the more common approach. Method selection also depends on the choice of the separation mode. Table 10.2. While chiral stationary phases are the only choice for gas chromatography [16,28,33-38], and are used almost exclusively for supercritical fluid chromatography [39-43] and capillary electrochromatography [44-47], they also dominate the practice of liquid chromatography... 

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