Enantioselective supercritical fluid chromatography

Svensson, L.A. and Owens, P.K., Enantioselective supercritical fluid chromatography using ristocetin A chiral stationary phases. Analyst, 125, 1037, 2000. 

This review provides an overview of the literature published to date on macrocyclic antibiotics exploited for enantioselective separations in high-performance liquid chromatography (HPLC). It was not intended as a comprehensive issue on the applications of such antibiotics in sub- and supercritical fluid chromatography (SFC), thin layer chromatography (TLC), capillary electrophoresis (CE), and capillary electrochromatography (CEC). A number of structural properties of the most important macrocyclic antibiotics applied in HPLC enantioseparations are listed in Table 2.1. 

Enantioselective separation by supercritical fluid chromatography (SFC) has been a field of great progress since the first demonstration of a chiral separation by SFC in the 1980s. The unique properties of supercritical fluids make packed column SFC the most favorable choice for fast enantiomeric separation among all of the separation techniques. In this chapter, the effect of chiral stationary phases, modifiers, and additives on enantioseparation are discussed in terms of speed and resolution in SFC. Fundamental considerations and thermodynamic aspects are also presented. 

Villeneuve, M.S., and Miller, L.A. (2005) Preparative-scale supercritical fluid chromatography, in Preparative Enantioselective Chromatography, 1st edn (ed. G.B. Cox), Wiley-Blackwell,... 

Phinney K. W, Sander, L. C. Additive concentration effects on enantioselective separations in supercritical fluid chromatography Chirality, 2003,15, 287-294. 

Chen, J. et al., Supercritical fluid chromatography-tandem mass spectrometry for the enantioselective determination of propranolol and pindolol in mouse blood by serial sampling, Anal. Chem., 78(4), 1212, 2006. 

Zeng, L. et al., Parallel supercritical fluid chromatography/mass spectrometry system for high-throughput enantioselective optimization and separation, J. Chromatogr. A, 1169(1-2), 193,2007. 

Kot, A. Sandra, R Venema, A. Sub- and supercritical fluid chromatography on packed columns A versatile tool for the enantioselective separation of basic and acidic drugs. J.Chromatogr.Sci., 1994, 32, 439-448... 

A further evaluation of the CB-DNP was reported by Gahm of Amgen using supercritical fluid chromatography (SFC) [27]. He concluded that inclusion com-plexation was the primary factor in retention and enantioselectivity. Better enantioseparation, as well as peak shape together with the reversal of elution order, makes CD-based CSPs unique and complimentary to the more commonly used jt-basic amylose and cellulose columns. 

Toribio L, Del Nozal Ml, Bernal JL, Alonso C, Jimenez JJ (2005) Comparative study of the enantioselective separation of several antiulcer drugs by high-performance liquid chromatography and supercritical fluid chromatography. J Chromatogr A 1091 118-123... 

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