Summary of Calibration and Validation Procedures

There is no single standard procedure for Raman spectrometer calibration, since instrument configurations and applications vary so widely. Nevertheless, it is useful to summarize typical procedures, some of which resulted in the spectral results presented in this chapter. 

Once a new instrument is installed by the vendor or set up for the first time, several benchmark measurements should be conducted and saved for future reference  

After frequency calibration by a recommended (or established) procedure, obtain and store a spectrum of an ASTM standard, such as cyclohexane or 4-acetamidophenol. 

Repeat acquisition of the standard over a period of several minutes to test short-term frequency reproducibility. 

Repeat acquisition of the standard over several days, using whatever frequency calibration technique and interval are to be used in real applications. 

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