Sulfotransferase induction

Induction of DNA single-strand breakage in rat liver after in-vivo exposure to N-nitrosodiethanolamine was demonstrated in three studies and dose-dependent effects were shown. In one of these studies, the DNA strand-breaking potential of 7V-nitroso-diethanolamine was found to be abolished by inhibition of sulfotransferase by 2,6-dichloro-4-nitrophenol. Unscheduled DNA synthesis was not detected in rats or mice in an in-vivo/in-vitro hepatocyte DNA repair assay after treatment with 7V-nitrosodi-ethanolamine. A single study in mice exposed in vivo to 7V-nitrosodiethanolamine did not find any significant induction of structural or numerical chromosomal aberrations or micronuclei in bone-marrow cells. 

Fiala, E.S., Sodum, R.S., Hussain, N.S., Rivenson, A. Dolan, L. (1995) Secondary nitroalkanes induction of DNA repair in rat hepatocytes, activation by aryl sulfotransferase and hepatocarcinogenicity of 2-nitrobutane and 3-nitropentane in male F344 rats. Toxicology, 99, 89-97... 

Curcumin has also been found to interrupt the cell cycle, to have cytotoxic effects, and to have a role in antiproliferation and the induction of apoptosis in a hepatocarcinoma cell line. Curcumin is a potent inhibitor of phenol sulfotransferase (SULT1A1) in human liver and extrahepatic tissues [Vietri et al., 2003]. Curcumin inhibited the interleukin-6 (IL-6) production, histone acetyltransferase (HAT) activity, and API activation [Chen et al., 2003a] and prevented cell death and apoptotic biochemical changes, such as the... 

Cytosol easy to use, cheap addition of cofactors (simple mixtures), Only not membrane-bound metabolizing enzymes such as alcohol dehydrogenases, sulfotransferases, glutathione S transferase, N-acetyl transferases partial metabolic profile, induction not modeled... 

Table 4 Drug metabolizing enzyme (P450 isoforms (CYP), UDP-glucuronosyl transferase (UGT) and phenol sulfotransferase (PST)) substrates that can be used for the in situ measurement of activity in the human hepatocyte enzyme induction assay.

Duanmu, Z., Dunbar, J., Falany, C. N., and Runge-Morris, M. (2000) Induction of rat hepatic aryl sulfotransferase (SULT1A1) gene expression by triamcinolone acetonide impact on minoxidil-mediated hypotension. Toxicol. Appl. Pharmacol. 164, 312-320. 

B. (2001) Dehydroepiandrosterone sulfotransferase gene induction by bile acid activated farnesoid X receptor. J. Biol. Chem. 276, 42549 -2556. 

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