Sulfonamides liquid chromatographic methods

Horie et al. (247) described a multiresidue liquid chromatographic method for the determination of 10 sulfonamides in honey. According to this method, 5 g honey is dissolved into 50 ml 30% sodium chloride and extracted with 60 ml dichloromethane. The extract is dried with anhydrous sodium sulfate and applied to a Sep-Pak Florisil cartridge. Following cartridge washing with 10 ml... 

Tsai, C.-E. Kondo, F. A sensitive high-performance liquid chromatographic method for detecting sulfonamide residues in swine serum and tissues after fluorescamine derivatization. J.Liq.Chromatogr,... 

AO AC, Sulfonamide residues in raw bovine milk- liquid chromatographic method, 993.32, in Official Methods of Analysis, 18th ed. (revised), AOAC International, Gaithersburg, MD (available at http //www.eoma.aoac.org/ gateway/readFile. asp id=993 32. pdf accessed 3/24/10). 

Following their extraction and cleanup, residues of sulfonamides and diaminopyrimidine potentiators in sample extracts can be detected by direct nonchro-matographic methods, or thin-layer, gas, liquid, or supercritical fluid chromatographic methods (Table 29.7). 

Long, A.R. Short, C.R. Barker, S.A. Method for the isolation and liquid chromatographic determination of eight sulfonamides in milk. J.Chromatogr., 1990, 502, 87-94... 

Sulfonamides having a free j )-amino group are readily assayed by titration with nitrous acid. The sulfonamide function may also be titrated with base, such as lithium methoxide. The majority of the sulfas listed in the U.S. Pharmacopeia XXII, however, are assayed by chromatographic methods, particularly high performance liquid chromatography (49). Sulfonamides for which assays are listed in the U.S. Pharmacopeia XXII-National Formulary XVII include the following sulfacetamide, sulfabenzamide, sulfadiazine, sulfadoxine, sulfamerazine, sulfamethazine, sulfamethizole, sulfamethoxazole, sulfapyridine, sulfasalazine, sulfathiazole, sulfinpyrazone, sulfis ox azole, sulfisoxazole acetyl, sulfisoxazole diolamine, sulfoxone, triple sulfa, dapsone, and various combinations with prednisolone, pyrimethamine, and trimethoprim. 

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