Sulfated polysaccharides techniques

De Zoisa et al. (2008) reported the isolation and characterization of fucose-containing sulfated polysaccharide as an anticoagulant agent from the edible brown seaweed Sargassum fulvellum by means of a simple fermentation process and chromatography technique. According to their... 

Our experimental approach is mainly based on the use of mlcr calorimetric, potentlometric, and chirooptical techniques. Polymers considered include 1] sulfated polysaccharides (i-carragee-nan, dextran sulfate] ... 

ESI-MS/MS analysis. Also Becker et al. employed a combination of NMR with chemical analysis and additional biophysical techniques to deduce the structure of 2-sulfated, 3-linked ot-L-galactan and a-L-fucans.46 It was indicated that the extremely different conformations of the two polysaccharides could help to explain their distinct anticoagulant properties. 

The hydrodynamic resistance of solutions of connective-tissue polysaccharides has been measured by sedimentation techniques [9] the resistance is expressed as the hydraulic conductivity (k), which was defined in Equation 6-9 (Figure 6.8). This analysis permitted the identification of two different groups of polysaccharides those compounds containing Pi 4- and y6i 3-link-ages (such as chondroitan sulfate, keratan sulfate, and hyaluronate) had lower hydraulic resistance than compounds containing some fraction of... 

The achievement of a successful analysis depends not only on optimized analytical procedures but also on (1) the ability of the extraction procedure to recover DNA from the sample and to remove potential assay inhibitors, and (2) the quality and purity of the DNA extracted. The analytical technique is useless if the target cannot be extracted from the sample. At this point in time there are no standardized procedures for the extraction of DNA from food samples. The development of DNA extraction procedures is matrix dependent. High fat content and low dry matter seem to explain the decreased extraction efficiency of full-fat soybean flour compared to its defatted counterpart (Gryson et al., 2008). Traditionally, the extraction of DNA has been carried out by treating the sample with detergents such as sodium dodecyl sulfate and proteinases such as proteinase K, followed by the removal of proteins and polysaccharides with phenol-chloroform and precipitation of DNA with ethanol. 

Ammonium sulfate salt precipitation is a classic method used to concentrate and partially purify various proteins, for example, diphtheria and tetanus toxins. Alcohol precipitation is effective for separating polysaccharides from proteins. Cohn cold alcohol precipitation is a classic technique used to fractionate blood serum for antibody isolation. Both techniques concentrate antigen for further treatment. 

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