Sugar, detection

Fehling s solution (sugar detection and estimation) (a) Copper sulfate solution dissolve 34.639 g of CUSO4 5H2O in water and dilute to 500 mL. (b) Alkaline tartrate solution dissolve 173 g of rochelle salts (KNaC40g dHjO) and 125 g of KOH in water and dilute to 500 mL. Equal volumes of the two solutions are mixed just prior to use. The Methods of the Assoc, of Official Agricultural Chemists give 50 g of NaOH in place of the 125 g KOH. 

Thinking Critically Were reducing sugars detected in the hydrolyzed starch solution using the Benedict s test Was this expected ... 

Horie S, Kubo Y (2009) Fluorescence-based indicator displacement assay for phospho-sugar detection using zinc(II) dipicolylamine-appended phenylboronic acid. Chem Lett 38 616-617... 

An alternative approach, which additionally favors the separation of sugars from interfering compounds, is the precolumn formation of the derivatives in this case trace sugar detection is sometimes possible, as nmol or pmol orders are approached. Examples include the use of dan-sylhydrazine (with either fluorimetric or colorimetric detection) (39) and l-phenyl-3-methyl-5-pyrazolone (UV absorption or electrochemical detection) (40). 

Among the other sugars detected in the soil, only the 6-deoxyhexoses have been quantitatively determined. In Delaware soils, rhamnose and fucose, determined by quantitative paper-chromatography, amounted to 20% of the sugars. Under the conditions of furfural formation from pentoses, the 6-deoxyhexoses yield 5-methyl-2-furaldehyde this has been determined by the differential solubilities of the phloroglucides in alcohol. The proportion of 6-deoxyhexoses in some cases exceeded that of pentoses. 

Table II. Constituent Sugars Detected on Paper Chromatograms of Acid Hydrolyzate of Pseudomonas Cellulases A, B, and C...

ORIGIN/INDUSTRY SOURCES/USES found as a free base in natural waters manufacture of azo dyes intermediate in synthesis of various colors plastic films determination of nicotine (quantitative) hardener in rubber compounds reagent spray reagent for sugars detection of hydrogen cyanide and sulfate detection of blood stains lignification measurement... 

Sugars detected as Sugars detected as Structural alditol acetates alditol acetates unit... 

Amino Acid and Amino Sugar Detected in Add Hydrolyeates of Bacterial Ceil Walls... 

The complexation of aldoses with cholesterol-derived boronic acids has been reviewed, and the changes in UV absorption and fluorescence intensity of a stilbene-type boronic acid on complexation with aldoses, especially D-fructose, have been studied with a view to their use in sugar-detection. Similar work has been carried out with diboronic acids derived from biphenyl and a -functionalized diaza-18-C-6 crown ether.A carbohydrate boronic acid derivative with liquid crystal properties referred to in Chapter 6, and further reports on boronate esters are noted in Chapter 17. 

Hamaji, I. Protein for fluorescent sugar detection and its production method. Jpn. Kokai Tokkyo Koho JP 2000338044, 2000. 

Intramolecularly coordinated. Nmr shift reagent for flavonoids spray reagent for sugar detection after chromatog. 

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