Sugar analyses, methods

Polarization is the most common method for the determination of sugar in sugar-containing commodities as well as many foodstuffs. Polarimetry is apphed in sugar analysis based on the fact that the optical rotation of pure sucrose solutions is a linear function of the sucrose concentration of the solution. Saccharimeters are polarimeters in which the scales have been modified to read directiy in percent sucrose based on the normal sugar solution reading 100%. 

ICUMSy Methods Book, International Commission for Uniform Methods of Sugar Analysis, Norwich, U.K., 1994. 

ANALYTICAL METHOD FOR SUGAR ANALYSIS 10.10.1 Quantitative Analysis... 

Before discussing these most commonly applied tests in detail, certain general principles should be presented. Converting a macro-method to a micro-method sometimes involves no more effort than decreasing all the volumes used in the procedure. This general procedure is well known to clinical chemists. A typical example would be the Folin method for doing sugar analysis, where a final volume of 25 ml was obtained. 

Environment Canada, 18 542 23 120 Enzymatic cellulose hydrolysis, 26 359 Enzymatic hydrolysis, 10 503, 535-536 Enzymatic methods, in sugar analysis, 23 475-476... 

ICI Leading Concept Methanol (LCM) process, 76 305 Icon, formulation, 7 564t ICUMSA tables, 23 474. See also International Commission for Uniform Methods of Sugar Analysis (ICUMSA)... 

Somogyi method, in sugar analysis, 23 475 Sonication, 16 410-411 Sonic limit, heat pipe, 13 230 Sonogashira coupling... 

C. A. Browne and F. W. Zerban Physical and chemical methods of sugar analysis Third Ed., John Wiley and Sons, Inc., New York, 1941. 

Phosphorus, fatty acids, carbohydrates, glycerol, and amino acids were analyzed by the method described in our previous paper [8] and references cited therein. SDS-PAGE [8], TLC [9], HPLC [9], determination of phos-phomonoester [8], reducing sugar analysis [13], methylation analysis [14], and hexose analysis [15] were performed as described in the respective literature. Two dimensional TLC was performed on silica-gel plate (Merck Silicagel 60 F254 No. 5715) using the solvent systems, chloroform-methanol-acetic acid (65/10/1, v/v/v) for the first development and chloroform-methanol-25% ammonia solution (65/10/1) for the second. 

For this purpose, we cleaned the lower than 1000 cm and higher than 1500 cm" areas by subtracting the Raman spectrum of the solvent (see Analysis Methods) in order to make some possible modifications of the sugar-phosphate part of DNA upon complexation conspicuous. Furthermore, we could expect to refine the nature of the weak interactions of SOAz with DNA b s by observing the low frequencies characteristic of these bases. 

Analysis of honey on this continent dates back to 1892, when 500 commercial samples of honey were analyzed.50 The analytical methods used were, with certain modifications, employed over the years by several workers,51-54 until a critical study of the methods of sugar analysis was made by White and coworkers.55... 

There are several good methods for sugar analysis. Linkage analy-... 

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