Sucrose phosphorylase bacterial

TRETHEWEY, R.N., FERNIE, A.R., BACHMANN, A., FLEISCHER-NOTTER, H., GEIGENBERGER, P, WILLMITZER, L., Expression of a bacterial sucrose phosphorylase in potato tubers results in a glucose-independent induction of glycolysis, Plant Cell Environ., 2001, 24, 357-365. 

The dried bacteria also contain invertase and this hydrolytic enzyme competes with the sucrose phosphorylase for sucrose. However, it is possible to eliminate most of the invertase from the bacterial preparations by several precipitations with ammonium sulfate. Using a partially purified sucrose phosphorylase preparation and a mixture of... 

Sucrose phosphorylase is a bacterial enzyme that catalyzes the phosphorolysis of sucrose to yield glucose-1-phosphate and fructose. 

Doudoroff, M., Barker, H. A., and Hassid, W. Z., 1947, Studies with bacterial sucrose phosphorylase. III. Arsenylated decomposition of sucrose and glucose-l-phosphate. J. Biol. 

The in vitro enzymatic synthesis of sucrose was accomplished by Hassid, Doudoroff, and Barker 100), They found that a bacterial preparation from Pseudomonas saccharophila contains a phosphorylase capable of catalyzing the reversible reaction ... 

When cultures or enzyme preparations of N. perfiava are allowed to act on a-D-glucose-l-phosphate, some amylopolysaccharide is produced, indicating the presence of a phosphorylase. However, the amylosucrase can be distinguished from the bacterial phosphorylase by its stability to heat and by the fact that the synthesis of the polysaccharide from sucrose is not suppressed in high concentration of inorganic phosphate. The synthetic reaction proceeds without a noticeable lag period, suggesting that primer material, if needed, is present in the preparations. Although there is no direct evidence for this, the fact that the formation of polysaccharide is completely inhibited in the presence of traces of salivary amylase indicates that a primer may be required. 

In green tissues Smith (320) showed sucrose rather than starch to be formed in brief photosynthesis, and in their experiments with C H)s Calvin and Benson (69) found that sucrose became labeled when no labeled glucose or fructose could be detected. The conclusion was drawn that sucrose arose from G-6-P and F-6-P, rather than from the free sugars. Although the synthesis of sucrose carried out by bacterial enzymes described by Hassid el al. (149) resembles the phosphorylase reactions described above, a different mechanism of sucrose synthesis is found in plants. Recent work by Buchanan (57) and by Leloir and his co-workers (73,223,224) has shown the following reactions leading to sucrose synthesis ... 

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