Triantennary structure

Scheme 13.18 JV-linked glycopeptide with a triantennary structure.

Figure 14.3 Structure models of four different conformer populations (2 for each 1—>6 link) of a triantennary high-mannose undecasaccharide M9. Terminal mannoses are highlighted as CPK models.12...

The triantennary glycan structure of the membrane glycoprotein of vesicular stomatitis virus (New Jersey serotype) has been elucidated by Ballou and coworkers215 (see Fig. 38). 

Fig. 2. iV-glycans of the hybrid type. A triantennary structure with sialylated Af-acetyllactosamine (LacNAc) building blocks B biantennary structure with sialylated or fucosylated poly-LacNAc building blocks. The N-glycan core structure is written in bold letters... 

It must be emphasized that the extension of a triantennary structure with a NeuAc group a-(2— 6)-linked to Gal-8 can be unambiguously established on the basis of both the unique set of chemical shifts for its H-3c, H-3e, and N-acetyl protons, as well as its specific effects upon the chemical shifts of the structural-reporter groups of GlcNAe-7 and Gal-8 (compare Table XIII). 

The triantennary type of structure of 53 may be derived from comparison of the chemical shifts of the H-l and H-2 signals of Man-3, -4, and -4 with those of 9 and 48 (see Table VI). The structural-reporter-group signals for the upper (4-5-6-NeuAc) and lower (4 -5 -6 -NeuAc ) branch residues are in accord with those reported for compound 34 (see Table X). 

Hybrid oligosaccharides having zero and one sialic acid were also present. Biantennary structures having one sialic acid, both with and without fucose, and a triantennary structure were detected as well. Asp-N peptide 20.7 contained many of the same structures (Fi es 6,7) as in peptide 20.3 with the addition of a biantennary with no sialic acid and a hybrid structure. Asp-N peptide 20.9 (Figure 8) contained many of the same structures in the above peptides with the addition of a biantennary structure with two sialic acids. Many of the same structures were detected in most of the peptides due to the poor chromatographic resolution of these peptides in the pepti map. 

B) Labelling of glycans Radioactive labelling oligomannose and hybrid structures, Endo F2 digests oligomannose and biantennary structure and Endo F3 is specific for bi- and triantennary glycans. Classical method, sensitivity of ... 

Fig. 6. Primary structure of serotransferrin diantennary glycans from human (A,B) [211-213], cow (A,B) [119, 214], rabbit (A) [103], sheep (A) [119,215], marsupial (kangaroo, opossum, wallaby) (A) [119,216], Primary structure of human serotransferrin triantennary glycans (C,D) [119-217],...

Fig. 5. Oligosaccharide structures for mouse PrP at AsnlSO and 196 (equivalent to 181 and 197 in hamster and human), obtained by Stimson et al. (26), grouped as (1) biantennary, (11) triantennary, and (111) tetraantennary. Squares, A -acetylglucosamine open circles, mannose closed circles, galactose triangles, fucose ovals, sialic acid. (Reproduced with permission from Stimson, E. et al. [1999]. Biochemistry 38, 4885-4895. Copyright American Chemical Society.)...

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