Structure of Antibody

Alzari, P.N., Lascombe, M.-B., Poljak, R.J. Three-dimensional structure of antibodies. Annu. Rev. Immunol. 6 SSS-S80, 1988. 

Colman, P.M. Structure of antibody-antigen complexes implications for immune recognition. Adv. Immunol. 43 99-132, 1988. 

Alzari P.M., Lascombe M. Poljak R.J. (1988) Three-dimensional structure of antibodies. Ann Rev... 

Guichard, G., Briand, J. P., Muller, S., Cung, M. T. Structure of antibody-bound peptides and retro-inverso analogues. A transferred nuclear Overhauser effect spectroscopy and molecular dynamics approach. Biochemistry 2001, 40, 5720-5727. 

Recentiy published crystal structures of antibody 4C6, an antibody that catalyzes another cationic cyclization reaction (Figure 6), revealed that this antibody has exquisite shape complementarity to its eliciting hapten 5. The active site contains multiple aromatic residues which shield the high-energy intermediate from solvent and stabilize the carbocation intermediates through cation-7r interactions. 

Anti-glycosyl antibodies isolated from the sera of some animals immunized with bacterial carbohydrate antigens are of a restricted heterogeneity, that is, they occur in relatively few, multimolecular forms.145 146 Such antibodies have been used in studies on the structure of antibodies,147 and on the genetic regulation of synthesis of anti-... 

Gerald M. Edelman and Rodney R. Porter Physiology/Medicine Structure of antibodies... 

Fig. 3.1 The structure of antibodies. H-chain heavy chain, consisting of VH, CHl,CH2andCH3 L-chain light chain, consisting of VL and CL ...

R.J. Poljak. 1991. Structure of antibodies and their complexes with antigens Mo/. Immunol. 28 1341-1345. (PubMedl... 

We used several methods were used to model antibody CDRs that can be divided into two categories (1) a knowledge-based approach that uses CDRs from known crystal structures of antibodies and (2) an ab-initio approach that builds CDR loops. All of these approaches must fulfill one criterion to identify the conformation that is best adapted to the framework of a current model. Unfortunately, none of the current methods has an... 

Precipitation with salts, organic solvents and polyethylene glycol (PEG) has often been employed as an approach for fraction separation, which usually attracts the water essential for maintaining the 3D structure of antibody molecules, so that they precipitate [5]. Ammonium sulphate is a common salt used for antibody precipitation [125], but since it results in high levels of unspecific binding (i.e., 5-20%), it is seldom used nowadays for fraction separation purposes. 

Scheme 20. Transition state structure of antibody-14D9 catalyzed enantioselective protonation...

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