Structure-Guided Pyruvate Aldolase Modification

Structure-guided site-directed mutagenesis offers a great potential for the rapid preparation of mutants with new/improved activity when the structure of the enzyme and its catalytic mechanism are known [29,111-114]. 

To overcome the poor stereoselectivity of KDGlu a pair of stereocomple-mentary KDGlu variants were obtained for the dol addition of pyruvate to 

10 RECENT ADVANCES IN ENZYME-CATALYZED ALDOL ADDITION REACTIONS 

Stereodivergent synthesis of 4S- and 4/ -configured 6-dialkylaminocarbonyl /V-acetyl-neuraminic acid derivatives (53 and 54), catalyzed by NeuA E192N/T167G (NeuA =) 

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