Structure Determination of Oligosaccharides by Sequential Digestion

TABLE 11.3. Commonly Used E oglycosidases for Sequential Degradation of Oligosaccharides 

Tandem Mass Spectrometry for Structural Analysis cf Carbohydrates 

the miz value of these sequence-specific ions is calculated as follows Positive-ion mode  

Metal-Ion Adducts as Precursor Ions CID of the metal-ion adducts has been a useful tool to distinguish linkage positions in oligosaccharides. These applications have inclnded cationization with alkali [9,20,21,32], alkaline earth [32], and transition metals [33]. 

ESI In-Source CID of Oligosaccharides In-source CID can also provide information on linkage and anomeric configurations [13,46]. Derivatization improves HPLC separation and fragmentation [46]. This aspect was demonstrated by the ESI in-source CID of / -aminobenzoic acid ethyl ester-labeled disaccharides and oligosaccharides [46]. 

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