Strobe method

Figure 7.6 Schematic profile of the fluorescence lifetime measured by the Strobe method. Courtesy of Jobin Yvon.

Beckman Elutriation Method. The Beckman elutriation method uses a chamber designed so that the centrifugal effect of the radial inward fluid flow is constant (Fig. 3). The separation chambers are made of transparent epoxy resin which faciUtates observation of the movements of the cell boundary in strobe light illumination. This enables detection of the radius at which the cells are separating. When a mixture of cells, eg, mononuclear white cells, enters the chamber, separation can be achieved by fine tuning centrifuge speed and inward fluid flow to the specific cell group. This is a laboratory method suitable for relatively small numbers of cells. Chambers are available in sizes to handle 2-3 x 10 , 1 2 x 10 , and 1 x 10 ° cells. The Beckman chambers can be appHed to collect mononuclear cells from bone marrow aspirates. 

Table 2.4. Fluorescence lifetime of fluorophores measured with Strobe, the phase and TCSPC methods.

Determine the flow measurement method to be used during the test, i.e., flowmeter or pitot tube measurement from the nearest available water outlets on the system. Ensure the flow measurement devices are calibrated or adjusted for the fire pump maximum flow output. Ensure independent measurement devices for verification of driver (i.e., engine) and pump rpm are available and accurate, i.e., strobe light handheld tachometer. 

Quiescent "current saving" method strobes LEDs 160+ Hz. To achieve <10% duty cycie to prolong battery life... 

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