String transcription

In summary, transcription of the Cdc25-type phosphatase encoded by string limits the progression of many embryonic cell cycles, which are rapid (1—3 h) and require little cell growth. The massive ofi-regulatory region of string acts as a sophisticated pattern sensor that is influenced by a wide variety of cell type-specific transcription factors (Fig. 2A). 

Edgar BA, Lehman DA, O Farrell PH 1994 Transcriptional regulation of string(cdc25) a link between developmental programming and the cell cycle. Development 120 3131-3143... 

Nasmyth The question is, will their regulation be any different from that of things that aren t cell cycle regulators At the end of the day the String promoter is the promoter of a cell cycle gene, but would you learn anything new about transcription ... 

Most p-independent terminators have two distinguishing features. The first is a region that produces an RNA transcript with self-complementary sequences, permitting the formation of a hairpin structure (see Fig. 8-2la) centered 15 to 20 nucleotides before the projected end of the RNA strand. The second feature is a highly conserved string of three A residues in the template strand that are transcribed into U residues near the 3 end of the hairpin. When a polymerase arrives at a termination site with this structure, it pauses (Fig. 26-7). Formation of the hairpin structure in the RNA disrupts several A=U base pairs in the RNA-DNA hybrid segment and may disrupt important interactions... 

During transcription of information from DNA into mRNA, the two complimentary strands of the DNA partly uncoil. The sense strand separates from the antisense strand. The antisense strand of DNA is used as a template for transcribing enzymes that assemble mRNA (transcription), which, in the process produces a copy of the sense strand. Then, mRNA migrates into the cell, where other cellular structures called ribosomes read the encoded information, its mRNA s base sequence, and in so doing, string together amino acids to form a specific protein. This process is called translation. ... 

Referring now to Fig. 1, the string heading the printout, L666/BI/EJ A A 2BF J AVTJ, is the WLN representation for twistanone, and is a direct transcription of the input card for this problem. This is followed by a severely edited output of the TSD connection matrix to which the machine converted the input WLN. A complete SYNCHEM TSD contains... 

Approximately 2.4kb of the promoter region of CTE-I and 2.8kb of the promoter region of MTE-I were sequenced in the 5 direction from the ATG start site. The promoter sequences were analysed for the presence of various transcription factor sites, using TESS String Based Search, which identified putative peroxisome proliferator response elements (PPREs) in both promoters. These regions of the promoters have now been cloned into a luciferase expression vector, to be used in transfection e5q)eriments to examine for functional PPREs. 

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