Streptomycin production media

Saval S, Pablos L, Sanchez S (1993) Optimization of a culture medium for streptomycin production using response-surface methodology. Bioresour Technol 43 19-25... 

Medium Time of harvest days Average wet weight of mycelium g/flask T ransamidinase Streptomycin production mg/g mycelia... 

Method. Figure 3 shows the equipment used by us for loading a culture medium with radon and decay products. Air was circulated in a closed system, driven by a membran pump (MP). The system consisted of a Ra-226 solution (Ra), a security bubble flask with water (H20), a membran bacteria filter (MF) and a second bubble flask containing 100 ml RPMI 1640 culture medium (CM). This medium contains 100 IE/ml penicilin and streptomycin and 0.75% L-glutamin. Foetal calf serum, an essential part of blood cultures, must not be added, else the airstream would develop foam. Furthermore we added a small amount of Pb(N03)2 and Bi(N03)2, about 10 ng of each, as "carriers" for the radon decay products to avoid a "wall effect". 

Media basal RPMI 1640 medium requires supplementation with sodium pyruvate, L-glutamine, and penicillin/streptomycin before use. The supplements are supplied as concentrates of 50X or 100X, and the appropriate amounts should be added. Standard tissue culture media for hybridoma production contain 5%, 10%, or 15% fetal bovine serum (FBS see Note 6). Sufficient quantities should be prepared in advance and a sterility check should be performed on them prior to use. 

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