Streptomycin 6-phosphate, biosynthesis

The poly (ribitol phosphate) synthetase and poly (glycerol phosphate) synthetase are inhibited by vancomycin, novobiocin, and Crystal Violet. Other antibiotic substances which interfere with cell-wall synthesis (such as bacitracin, ristocetin, and streptomycin) are almost without effect on the isolated synthetases, and penicillin is inhibitory at high concentrations only. Moreover, penicillin, vancomycin, and bacitracin do not markedly inhibit synthesis of cell-wall glycosaminopeptide in vitro, although the synthetical activity of extracts of cells which have been pretreated with these antibiotics is lowered.Convincing evidence that the primary site of inhibition by antibiotics is the biosynthesis of cell-wall material has been obtained only for the penicillins and cycloserine, and it appears that the action of even those antibiotics may be more complex than was originally supposed. 

S ATP -I- streptomycin (<1> streptomycin is secreted [1,2] <1> detoxification of a potent inhibitor of protein biosynthesis [3] <2> self-pro-tection of streptomycin producer, because the product streptomycin 6-phosphate does not inhibit protein synthesis [4]) (Reversibility [1-4]) [1-4]... 

Bioconversions of aminoglycoside antibiotics have been discussed. Further studies in the biosynthesis of streptomycin have shown that only streptidine-6-phosphate acts as an acceptor for dihydrostreptose in the synthesis of O-a-L-dihydrostreptose(l ->-4)streptidine-6-phosphate using a purified enzyme preparation from S. griseus, and that this pseudodisaccharide could be... 

Kniep B, Grisebach H. Biosynthesis of streptomycin. Enzymatic formation of dihydrostreptomycin 6-phosphate from dihydrostreptosyl strepridine 6-phosphate. J Amibiot 1980 33 416-419. 

Maier S, Grisebach H. Biosynthesis of streptomycin. Enzymic oxidation of dihydtostrepto-mycin (6-phosphatc) to streptomycin (6-phosphate) with a particulate fraction of Sirepto-... 

Walker JB, Skorvaga M, Streptomycin biosynthesis and metabolism. Phosphate transfer from dibydrostreptomycin 6-phosphate to inosamines, streptamine. and 2-deoxystreptamine. J Biol Chem 1973 248 2435-2440. 

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