Stopped fiow method technique

Following the original rapid-fiow experiments of Hartridge and Roughton, the introduction of the stopped-fiow method, and the use of electronic techniques for rapid recording,rapid-reaction techniques have found wide apphcation in chemistry and bio-... 

Under steady-state conditions, the cleavage of this substrate obeys Michaelis-Menten kinetics with a. ST of 20 pM and a A of 77 s f The initial phase of the reaction was examined by using the stopped-fiow method. This technique permits... 

Most substituents (Cl, Me, OMe) in the 2-position have only a small effect, if any, on the hydration of the quinazoline cation they are similar in this respect to substituents in the 5-, 6-, and 8-positions (see above). Although hydration in the 2-aminoquinazoline cation was at first considered absent, a closer examination of the entire spectra of both species indicated that the cation spectrum may be that of a mixture. Hydration in the cation has now been confirmed by the rapid-reaction technique (the stopped-fiow method) which showed that the unstable hydrated neutral species had a half-life of 4.0 sec at 20° and pH 9. 60. The 2-hydroxyquinazoline cation has not been studied, but... 

Table 2 Seiected appiications iiiustrating the advantages of the stopped-fiow technique over equiiibrium methods...

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