Staining total-periodic acid schiff

Fig. 6.3. SDS/PAGE (5-15% linear gradient gel) separated proteins of the total worm homogenate and isolated brush border fractions from protoscoleces of Echinococcus granulosus (horse strain), (a) Coomassie blue staining (b) Periodic acid-Schiff (PAS) staining. (After McManus Barrett, 1985.)...

Parenchymal cells (PC), or hepatocytes, originate from epithelial cells and represent most of the total number of liver cells (65%). Because of their relatively large size, hepatocytes are microscopically clearly visible after staining liver sections with hematoxylin and eosin (HE). Also, the hepatocytes store glycogen, which can be identified histochemically with periodic acid-Schiff (PAS) reagent. The hepatocytes are primarily responsible for the uptake of endogenous products and xenobiotics at the sinusoidal membrane of the cell and their subsequent metabolism and excretion into bile by means of the canalicular membrane. 

In addition to staining for total protein, electrophoretic gels can be used for detection of specific protein components. This can be done, for example, by treating the gel in conditions suitable to reveal enzymatic activities, such as chitinolytic (Vincenzi and Curioni, 2005), or staining for glycoproteins with the periodic acid-Schiff method (PAS) (e.g. Marchal et ah, 1996). 

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