Stabilization, A-DNA

Nymphaea caerulea, for seven natural anthocyanins stabilizing a DNA triplex, etc. Sequential analysis of the oligosaccharide structures of the flavonol tamarixetin-7-O-rutino-side has been performed by ID multistep-relayed COSY-ROESY experiments. Selective excitation was performed by Gaussian-shaped soft pulses. 

Fig. 1 Modified nucleic acids with enhanced nuclease stability, a DNA, b phosphorothioate, c Z -OjT-C-methylene-bridged (locked) nucleic acid (LNA), d 2f-0,A -C-ethylene-bridged nucleic acid (ENA), e Z -O-methyl nucleic acid, f methylphosphonate-linked nucleic acid, g morpholino-linked nucleic acid, h peptide nucleic acid (PNA)...

The structures and dynamics of water molecules in the DNA grooves play an important role in the biological function of DNA. As discussed later, the intercalation of the anti-cancer drug daunomycin into DNA is indeed facilitated by water molecules in the grooves. While the role of water molecules in stabilizing a DNA duplex is well known (water molecules screen the electrostatic repulsion between the negatively charged phosphate ions, form HBs with the polar atoms of the nucleic... 

Representing the effect of the environment Simulations of DNA under conditions expected to stabilize A-DNA. 

Schiff-base or imine bonds are also available as pH-sensitive crosslinkers. Because imine bonds are degradable at weakly acidic pH values, endosome-specific dissociation can be achieved by imine-based crosslinking. A simple imine-based crosslinker, glutaraldehyde, has been used to stabilize a DNA delivery system [111]. Photo-induced polymerization [112], epoxide reaction [113], and Michael addition [114] have also been reported as crossUnking methods. However, because... 

What are the consequences of these differences and do they hold any significance in common An argument can be made that, because of these differences, DNA is a more stable polymeric form than RNA. The greater stability of DNA over RNA is consistent with the respective roles these macromolecules have assumed in heredity and information transfer. 

The solute solvent contribution to the free energy stabilizing the DNA poly ion can be calculated within the polymer RISM theory by a charging up process " ... 

Ligands that interact physically with DNA have been extensively studied both by experimental techniques and by a variety of theoretical approaches. A diverse set of compounds have been studied, including compounds that intercalate between DNA sequences or bind in the minor groove.1 7 These studies have identified various factors that influence the stability of DNA ligand complexes in solution.6 8 9... 

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