Squash technique

Beeks, R.M. 1955. Improvements in the squash technique for plant chromosomes. Aliso 3 131-133. 

Two basic procedures have been developed for studies on mammalian chromosomes during the last decade the squash technique and the airdrying method. Both are direct adaptations to testicular material of similar methods used for somatic tissues. 

From 1956 28) 1964, the squash techniques were widely used for... 

Evans et al. 4) developed an air-drying technique for meiotic preparations from mammalian testes which avoids certain shortcomings of the squash technique, since it provides a large number of analyzable cells and allows the scoring of many cells per animal and per testis. Since the spermatocyte population from a single testis is thoroughly mixed, the samples of cells scored can be considered random and representative of the whole testis. Furthermore, as pointed out by Evans et the... 

H. Gardner and H. Punett, An improved squash technique for human male meiotic chromosomes Softening and concentration of cells mounting in Hoyer s medium. Stain Technol. 39, 245-248 (1964). 

Salivary gland chromosomes squash technique Simple method Protocol 18... 

Salivary gland chromosome squash technique Protocol 19... 

California red scales were obtained from a laboratory colony reared and maintained on banana squash. Squash was first used as a host for the mass pr uction of California red scale under laboratory conditions by Rush Bumgardner of the Orange Coimty Insectary, Anaheim, Calif. 12). The suitability of banana squash for this purpose was shown by Flanders IS). Lindgren et al. 15y 17) adapted the rearing methods of the red scale to the requirements of insecticide studies and developed techniques for using this species as a laboratory test insect. 

Young anther sacs were dissected and meiocytes squashed according to the technique of Beeks (1955). These were viewed with a Zeiss phase-contrast microscope. 

Sharma, G. K., Madhura, C. V., and Arya, S. S. (1990). Interaction of plastic films with foods. I. Effect of polypropylene and polyethylene films on fruit squash quality. ]. Food Sci. Technol. 27, 127-132. (In Linssen, J. P. H., van Willige, R. W. G., and Dekker, M.(2003). Packaging-flavour interactions. Ch. 8. In "Novel Food Packaging Techniques" (R. Ahvenainen, ed.), Woodhead Publishing Ltd., CRC Press, Boca Raton, pp. 144-171). 

A technique to produce samples consisting primarily of C o and C70 by fractional sublimation of carbon soot was found and used to produce solid films of these molecules. Film compositions were determined using a surface analytical mass spectrometric technique. Vibrational Raman spectra of the purified films were measured and vibrational lines of both Ceo and C70 are identified. A Cto line at 273 cm is observed, in agreement with theoretical predictions for the lowest frequency H, squashing" mode of Buckminsterfullerene. The two strongest C o lines, found at 1469 and 497 cm , can consistently be assigned to the two totally symmetric A, modes on the basis of their frequencies and measured depolarization ratios. 

Microencapsulated Cl fixed on the surfaces of metal parts forming immovable joints have found application in various industrial sectors. The interior of the shells is liberated when the parts are brought into contact and the capsules become squashed. This technique is used for antirust protection of rivet joints of the skin of airplanes. Microcapsules with Cl are carried, with minimum outlay, to the demanding corrosion sites of casing and drilling pipe strings at excavation sites of oil and gas deposits. The effective use of inhibited microcapsules has not been fully realized due to a number of reasons, including technical and economic ones. 

The salivary gland chromosome method of detecting newly induced rearrangements has been described in detail by Bauer et al, (1938), who used it to study the pattern and frequency of rearrangements induced in sperm as a function of X-ray dosage. Some improvements in techniques have occurred since the work of these investigators principally, these are (1) the use of siliconed coverslips to improve the spreading of the chromosomes as the nuclei are squashed and (2) the use of aceto-lactic-orcein... 

Smearing, squashing, and comminution are simple preparation techniques that make it possible to obtain extensive information quickly. Depending... 

Impacts. One of the most-studied sports has been tennis. The variables of racquet design, swing technique, ball design, and area of the strings used will all change the trajectory and speed of the tennis ball in play. Numerous other sports can be studied in terms of impacts, including baseball, squash, hockey, field hockey, and cricket. 

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