Spongiosis flank

Fig. 6. Sensitized guinea-pig. Reaction seen on the flank 24 hours after a single application of 0.1% DNCB in acetone Spongiosis...

It should be noted, however, that the histological lesions, especially the spongiosis, are not always seen. This is probably due to the thinness of the epidermis of the guinea pig s flank (2 to 3 cellular layers). 

We have repeatedly observed on the flank with acanthosis, in addition to spongiosis (Fig. 7), the presence of alterations cavitaires , already described in 1890 by Leloir as typical of eczema in man. 

We will not repeat the description of spongiosis since this has been described in the evolution of the lesions of the flank and nipple (see p. 14 and 15). 

After 8 applications, spongiosis appear on the epidermis of the flank and even more so on the epidermis of the nipple. A light dermal infiltrate of similar appearance is evident both on the flank and on the nipple there is a clear acanthosis on the flank, but its appearance on the nipple is difficult to note in view of the intensity of the eczematous, epidermal lesions. 

DNCB 0.1% (in acetone) on the left nipple weight approx. 250 g flank right nipple not previously treated spongiosis left nipple previously treated (i applications) spongiosis multiple acanthosis... 

In a second group of sensitized guinea pigs, some also exhibited this acanthosis. On the contrary, in the others which did not present an acanthosis of the flank, we found small but distinct spongiosis (Fig. 17). 

We observe spongiosis on the nipple even if we do not observe it on the flank of the same guinea pig. It is surprising that we did not find similar lesions in both the flank and nipple. This shows that the lesions do not always evolve in the same manner on the flank and nipple (N. Hunziker, 1962). 

Fig. 17. Guinea pig sensitized to PNDMA. Flank 14 hours after a single application of 1% PNDMA in olive oil no acanthosis, little spongiosis...

After 14 hours, contrary to what we observe on the flank, the epidermal lesions on the nipple are not very extensive. We see swollen cells and occasionally spongiosis or outlines of spongiosis. Exceptionally the epidermis is completely invaded by the cells of the dermal infiltrate. The infiltrate is more or less extensive and contains some eosinophils. The reaction with alkaline phosphatase shows positive cells in the infiltrate. 

In guinea pigs sensitized with CA, PA and PNDMA, spongiosis are less frequently found. They are present almost always on the nipple, but rarely on the flank. 

Fig. 26. Guinea pig non-sensitized passive transfer by serum from a guinea pig sensitized to CA. Flank 24 hours after a single application of CA 25% in dioxane little spongiosis...

Fig. 30. a Guinea pig sensitized to DNCB. Acanthosis of the flank (due to X-rays) 14 hours after a single application of O.T ) DNCB in acetone a few epidermal alterations, no spongiosis. b Guinea pig sensitized to DNCB. Acanthosis of the flank (due to crude acid) 14 hours after a single application of 0.1% DNCB in acetone spongiosis... 

However, we have often observed localized spongiosis on the nipple as well as on the flank of the guinea pig sensitized with DNCB and other simple chemical substances (N. Hunziker and coll., 1964) — (Fig. 6, 9, 10). 

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