Spirastrella spinispirulifera

Pettit, G. R., Herald, C. L., Cichacz, Z. A., Gao, F., Schmidt, J. M., Boyd, M. R., Christie, N. D., and Boettner, F. E., Isolation and structure of the powerful human cancer cell growth inhibitors spongist-atins 4 and 5 from an African Spirastrella spinispirulifera (Porifera), J. Chem. Soc., Chem. Commun., 1805, 1993. 

Spirastrella spinispirulifera (53), Hyrtios altrum (54), and Cinachyra sp. (55). These compounds are present as very trace metabolites. The first compound in the series, spongistatin 1 (Scheme 30), was reported from a Spongia sp. collected from the Republic of the Maldives (52). The isolation was bioassay-... 

In the isolation of spongistatin 4 (Scheme 31), the CH2CI2 fraction from 2409 kg of Spirastrella spinispirulifera was initially separated by HPLC employing a pilot scale HPLC system on a silica-gel column, 3 x 0.15 m, operated at 150 psi. This was followed by a series of Sephadex LH-20 separations and multiple HPLC steps using the following columns Merck RP-2, Prepex- RP-8, and LiChrospher 100 RP-18 to yield 10.7 mg of spongistatin 4 (53). 

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