Sperm cryopreservation species

Hundreds of references to sperm cryopreservation in aquatic species are available today, and this number grows each month. Many authors summarized published methods in critical reviews (see Table 3.1). Anyone attempting sperm cryopreservation for the first time is referred to these reviews as a starting point. They will find ample information on the procedure and the factors which should be considered in a given species. In this section, we will focus on the items most common in all aquatic species, and describe the steps of a cryopreservation procedure (Fig. 3.2). 

Table 3.1 Some reviews on sperm cryopreservation in aquatic species, other than in books... 

DONG Q X, HUANG c J and TIERSCH T R (2007) Control of sperm concentration is necessary for standardization of sperm cryopreservation in aquatic species Evidence from sperm agglutination in oysters. Cryobiology, 54, 87-98. 

Cryopreservation of gametes from aquatic species, either for routine use in hatcheries or for long-term cryobanking, has reached varied levels of development (Table 3.2) under the expertise of a world-wide scientific community (Fig. 3.3). Sperm is easily cryopreserved in almost all species, although spermatophores in crustaceans are stUl difficult to handle and to test for fertilization. Cryopreservation of oocytes and larvae in molluscs shows promise, although in fish it is still a field of active research. 

The book by Cabrita et al. (2008b) gives more detailed information on specific procedures for sperm and egg cryopreservation in marine and freshwater species. 

TiERSCH T R and JENKINS j A (2003) Biosecurity and regulatory considerations for transfer of cryopreserved sperm and early life stages of aquatic species, in Lee C-S and Bryen O (eds). Biosecurity in Aquaculture Production Systems Exclusion of Pathogens and Other Undesirables. Baton Rouge, LA World Aquaculture Society, 171-198. 

VIVEIROS ATM and GODINHO H p (2009) Sperm quahty and cryopreservation of BrazUian freshwater fish species a review. Fish Physiol Biochem, 35,137-150. 

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