Sperm cryopreservation methods and adaptation to hatcheries

Hundreds of references to sperm cryopreservation in aquatic species are available today, and this number grows each month. Many authors summarized published methods in critical reviews (see Table 3.1). Anyone attempting sperm cryopreservation for the first time is referred to these reviews as a starting point. They will find ample information on the procedure and the factors which should be considered in a given species. In this section, we will focus on the items most common in all aquatic species, and describe the steps of a cryopreservation procedure (Fig. 3.2). 

Germplasm cryobanking in zebrafish and Zebrafish, medaka Sperm analysis Robles et al., 2011 

Review Current status of sperm Aquarium model species High-throughput Yang and Tiersch, 

Sperm quality and cryopreservation of Characiformes, Gene banking in Viveiros and 

Brazilian freshwater fish species a review Siluriformes Brazil Godinho, 2009 

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