Species-specific primer

Olsen, K. H., Bjerselius, R., Petersson, E., etal. (2000). Lack of species-specific primer effects of odours from female Atlantic salmon [Salmo solar L.) and brown trout Salmo truttaL.). Oikos 88,213-220. 

Yoder, W. T., and Christianson, L. M. (1998). Species-specific primers resolve members of Fusarium section Fusarium. Taxonomic status of the edible "Quom" fungus reevaluated. Fungal Genet. Biol. 23, 68-80. 

Figure 2. Allele specific determination of single nucleotide polymorphisms (SNPs). (a) The determined SNP responsible for resistance is flanked by either allele specific primes (primer pair 1 and 3 or 1 and 4, respectively) or by species specific primers (primer pairs 1 and 2). (b) The allele specific (top) or species specific (below) amplified fiagments are examined by different methods (c) yes/no answer on an agarose gel stained with ethidiiun-bromide, or with fiagments were measured in real time PCR equipment using different chemistries imiversal dye Sybr Green, fiagment specific probes (top) or allele specific probes (below).

If species-specific primers are used to identify the origin of a sample and no PCR product has been obtained, a second PCR with universal primers should be performed... 

Several studies have reported on the isolation and identification of LAB and yeasts in boza however, to our knowledge, only the studies of Botes et al. (2006), Todorov and Dicks (2006) and Todorov (2010) used biomolecular approaches to identify these microorganisms. In these studies, the numbers of LAB isolated from three boza samples ranged from 9 x 10 to 5 x lO CFU/ml. Carbohydrate fermentation reactions and PCR with species-specific primers classified the isolates as Lactobacillus paracasei subsp. paracasei, Lactobacillus pentosus, Lactobacillus plantarum, Lactobacillus brevis, Lactobacillus rhamnosus, Lactobacillus fermentum, Leuconostoc lactis and Enterococcus faecium. 

Muir, A., Harrison, E., Wheals, A. (2011). A multiplex set of species-specific primers for rapid identification of members of the genus Saccharomyces. FEMS Yeast Research, 11, 552-563. 

It is no surprise then that nucleic acid synthesized in vitro according to this mechanism has the same base composition, the same physical properties (molecular weight), and even the same biologic activity (as a transformation factor) as the primer nucleic acid. The polymerase is not species specific primer nucleic acids of different species origin are multiplied indiscriminately by the enzyme from E. coU. 

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