Speciation reversed-phase chromatography

B. Do, S. Robinet, D. Pradeau and F. Guyon, Speciation of arsenic and selenium compounds by ion-pair reversed-phase chromatography with electrothermal atomic absorption spectrometry. Application of experimental design for chromatographic optimisation, J. Chromatogr. A, 918(1), 2001, 87-98. 

The most commonly used methods for speciation analysis of iodine in tissues and food are chromatographic techniques, such as anion exchange, size exclusion and reverse-phase chromatography, coupled with ICP-MS detection. 

The advantages are similar to those of RP chromatography, but ion pairing reversed phase chromatography offers higher flexibility and versatility. However, changes in the speciation of analytes are more probable when a counter-ion is added to the mobile phase. 

Mercury speciation in hair samples has been carried out using reverse phase chromatography following a digestion at room temperature." " Methyl and inorganic mercury were determined using this method in less than 7 min, which is a vast improvement on lengthy extractions used previously to remove methyl mercury from hair. 

Yang et al. [83] accomplished speciation of organotin compounds using reverse-phase liquid chromatography with inductively coupled plasma mass spectrometric detection. The separation was complete in 6min and detection limits were in the range 2.8-16pg of tin for various species. 

Thompson, J. J. and Houk, R. S., Inductively coupled plasma mass spectrometric detection for multielement flow injection analysis and elemental speciation by reversed phase liquid chromatography, Anal. Chem., 58, 2541-2548, 1986. 

Olayinka, K.O., Haswell, S.J. and Grzeskowiak, R. (1989) Speciation of cadmium in crab-meat by reversed-phase high-performance liquid chromatography with electrothermal atomisation atomic absorptive spectrophotometric detection in a model gut digestive system. J. Anal. At. Spectrom., 4, 171-175. 

Shibata, Y. and Morita, M. (1989) Speciation of arsenic by reversed-phase high performance liquid chromatography-inductively coupled plasma mass spectrometry. Anal. Sci., 5, 107-109. 

J. Zheng, M. Ohata, N. Furuta, W. Kosmus, Speciation of selenium compounds with ion-pair reversed-phase liquid chromatography using inductively coupled plasma mass spectrometry as element-specific detection. J. Chromatogr. A, 874 (2000), 55-64. 

J. M. G. Lafuente, M. L. F. Sanchez, A. Sanz-Medel, Speciation of inorganic selenium and selenoaminoacids by on-line reversed phase high performance liquid chromatography-focused microwave digestion-hydride generation-atomic detection, J. Anal. Atom. Spectrom., 11 (1996), 1163-1169. 

I. Feldmann, N. Jakubowski, D. Stuewer, C. Thomas, Speciation of organic selenium compounds by reversed-phase liquid chromatography and inductively coupled plasma mass spectrometry, J. Anal. Atom. Spectrom., 15 (2000), 371D376. 

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