Soxhlet apparatus, lipid extraction with

Garrett and Barger (1974) have described the use of a similar adsorption sampler made of thin teflon sheet that will collect surface lipids with very little entrained seawater. The sheet of teflon is held by clips in a circular holder and is touched on to the water surface. After sample collection, the teflon sheet can be cut up with scissors and placed in a Soxhlet apparatus for extraction. 

Powdered plant material can also be extracted in a Soxhlet apparatus, first with hexane, for example, to remove lipids and then with ethyl acetate or ethanol to obtain phenolics. This approach is not suitable for heat-sensitive compounds. 

Solvent Extraction Studies. Techniques necessary to detect lipid species extracted from in vitro and in vivo exposed Biomer were developed. Extractions with a nonpolar swelling solvent (e.g., CHC13) employing a soxhlet apparatus were performed to extract preferentially lipophilic biological components from Biomer or previously isolated neointimal samples. Experimentation demonstrated the high solubility in chloroform of polymer-derived monomeric and oligomeric components. A separation of lipids from these components was therefore required. 

Hence, if chemical signals mediate den location by neonatal prairie rattlesnakes, skin lipids would seem to be the best candidates. Odorous substances used in the experiments to be described next were obtained by extracting shed skins in a soxhlet apparatus with methylene chloride as a solvent. 

Normally, in the HPLC analyses of lipids, the sample is submitted to solvent extraction using a mixture of chloroform/methanol (2 1) and is directly injected into the HPLC/MS system. The extraction can be assisted with sonication, Soxtec, or Soxhlet apparatus. Interestingly, Romanus et al. [25] submitted the solvent... 

Doi described a chloroform extraction method. PHA is extracted with hot chloroform in a Soxhlet apparatus for over 1 hour. Then, PHA extracted is separated from lipids by precipitating with diethyl ether, hexane, methanol or ethanol. Finally, PHA is redissolved in chloroform and further purified by precipitation with hexane. Ramsay et examined the recovery of PHA from three different chlorinated solvents (chloroform, methylene chloride and... 

The purification of KL was conducted by extracting KL in the soxhlet apparatus for 6 h with n-pentane to remove lipophilic, nonlignin matters such as wax and lipids (Sun and Tomkinson, 2001). The precipitate was filtered and washed twice with pH 2 water to remove the excess n-pentane and non-lignin phenolic compounds, which may still remain after the pulping process. The purified KL was then dried further in the vacuum oven at 45°C for another 48 h. 

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