Sortase A

Weiner, E.M., Robson, S., Marohn, M., Clubb, R.T. (2010) The Sortase A enzyme that attaches proteins to the cell wall of Bacillus anthracis contains an unusueil active site architecture. Journal of Biological Chemistry, 285 (30), 23431-23441. 

The Gram-positive human pathogen Streptococcus pneumoniae is a frequent causal agent of pneumonia, otitis, and meningitis. Thirty percent of S. pneumoniae clinical isolates are piliated due to carriage of the rlrA operon coding for three sortases, a regulating element and the structural... 

Oh I, Yang W-Y, Chung S-C, Kim T-Y, Oh K-B, Shin J (2011) In vitro sortase A inhibitory and antimicrobial activity of flavonoids isolated from the roots of Sophora flavescens. Arch Pharm Res 34 217... 

Sortases are transpeptidases, which use the linear epitope LPXTjG as a recognition site within a target peptide or protein. The cleavage of the amide bond between the threonine and glycine residue (J.) results in an acyl-enzyme intermediate. Subsequently, the thioester intermediate is attacked by a nucleophilic co-substrate comprised of an N-terminal glycine residue in order to form a native peptide bond. Sortase A (SrtA) derived from Staphylococcus aureus is commonly used for site-specific N- and C-terminal... 

Besides transglutaminases and sortases, a variety of enzymes are able to catalyze site-specific protein modifications in order to introduce reactive... 

One-pot reactions were also carried out with immobilized enzymes. The synthesis of Le -antigen derivatives was performed with immobilized p4GalT and oi3FucT [124]. Both enzymes were immobilized via the action of Sortase A from Staphylococcus aureus. 

Ito, T., Sadamoto, R., Naruchi, K., Togame, H., Takemoto, H., Kondo, H., and Nishimura, S.-I. (2010) Highly oriented recombinant glycosyltransferases site-specific immobilization of unstable membrane proteins by using staphylococcus aureus sortase A. Biochemistry, 49, 2604-2614. 

Dieye, Y., Oxaran, V., Ledue-Qier, E, et al. (2010) Eunctionality of sortase A in Laclococcus lactis. Appl Environ Microbiol 76,7332-7337. 

Sortase A (SrtA) Ent. faecalis SrtA involved in initial bacterial (Guiton et al. 2009)... 

Guiton, P.S., Hung, C.S., Kline, K.A., et al. (2009) Contribution of autolysin and Sortase A during Enterococcus faecalis DNA-dependent biofilm development. Infect Immun 77, 3626-3638. 

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