Solvents and Chemical Methods of Detection

The experimental separation conditions quoted in the different chapters of this book apply also when the various compound classes are present as radioactively labelled substances. The fractionation may be effected by adsorption and/or partition on adsorbents by partition in reversed phase chromatography on hydrophobic layers  

Chemical test reagents can be applied also to thin-layer chromatograms which contain radioactive substances. In addition, various procedures for detection and quantitative determination of labelled compounds can be employed. Both chemical and radiometric methods of detection should always be used on the same thin-layer chromatogram this yields information about both the chemical and radiochemical purity of the sample studied. 

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