Soluble green fluorescent protein

Surface plastnon resonance Association constant Dissociation constant Hexaethylene glycol spacer Sambucus nigra agglutinin High-mobility-group transcriptional factor Soluble green fluorescent protein Calmodulin... 

Other reports show that intracellular expression of misfolded or unstable proteins can be dramatically improved by directed evolution. For example, directed evolution increased the expression of disulfide-containing antibody fragments in E. coli 50-fold, to reach a level of more than 0.5 g/L 212L The expression of a wide spectrum amidase of B. stearothermophilus in E. coli was improved 23-fold by two mutations 2101. And, in vivo fluorescence of the green fluorescent protein was improved 45-fold by increasing its solubility and native folding in E. co/t[1401. 

Cha H.J., Wu C.F., Valdes J.J., Rao G. and Bentley W.E. 2000. Observations of green fluorescent protein as a fusion partner in genetically engineered Escherichia colt monitoring protein expression and solubility, Biotechnol. Bioeng., 67, 565. 

A series of star DHBCs were evaluated for their ability to transfect hmnan cervical HeLa cancer cells with the modified plasmid pRLSV40, bearing the enhanced green fluorescent protein as the reporter gene [55]. The copolymers utilized were composed of PDMAEMA and PHEGMA blocks (where PDMAEMA is an ionizable block, while PHEGMA is a non-ionic water soluble block). The experimental data indicate a decreased toxicity for the star copolymer, compared to a reference PDMAEMA star homopolymer, for the same amounts of star polymer tested. Moreover, it has been found that the architecture of the star copolymer, i.e. star block, miktoarm star etc, plays a decisive role on the transfection efficiency. The best performance, for all star copolymers tested, was observed for a star block copolymer with... 

Endosialidases have been engineered to bind specifically o2,8-linked polysialic acid as inactive variants for fluorescence microscopy [69, 105, 168]. The endosialidase gene of the mutant phage K1A2 has been fused to green fluorescent protein (GFP). A repeat part of the Yersinia enterocolitica adhesin (yadA) stalk [199] was added between the endosialidase and GFP to increase the yield of soluble protein. A fusion protein containing the catalytically active form of the endosialidase prepared for control was negative [168]. 

In 1962, Olson and Romano isolated and purified a water-soluble BChl a-protein from green bacteria. Soon afterwards, the crystalline form of the protein was also obtained . It was in fact the first photosynthetic pigment-protein to be crystallized. Chemical studies showed the BChl a-protein to have a molecular weight of 150 kDa and to contain 21 BChl a molecules. It has a major absorption band in the far-red at 809 nm, which is ascribed to the Qy transition of BChl a, and a corresponding fluorescence band at 818 nm. Its circular-dichroism spectrum in the far-red absorption-band region shows multiple components which can be interpreted in terms ofexciton interactions between the BChl a molecules " . Fluorescence studies of the BChl a-protein in situ indicate that it functions as an intermediate in exd-tation-energy transfer from BChl c in chlorosomes to the BChl-dimer reaction center, P840 ... 

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