Sodium lactate injection, compound

Compound Injection of Sodium Lactate, P.P., Volhard 0 003546 g total Cl. 

Compound Injection of Sodium Lactate, B.P. A solution of lactic acid 0 25 per cent w v neutralised with sodium hydroxide, containing sodium chloride 0 6 per cent, potassium chloride 0 04 per cent, and calcium chloride 0-04 per cent. 

Metabolic blood parameters are assayed in anesthetized male rats using a modified method of glucose clamp studies in rodents (Terrettaz and Jeanrenaud 1983). Four to 6 rats per group (vehicle control and one dose of the candidate compound) are used. Rats are anesthetized with an intraperitoneal injection of pentobarbital sodium (60 mg/kg), tracheotomized, and one jugular vein per rat is cannulated for intravenous infusion the other vein is prepared for collection of blood samples. Anesthesia is maintained for up to 7 hours by subcutaneous infusion of pentobarbital sodium (adjusted to the anesthetic depth of the individual animal about 24 mg/kg/h). Body temperature is monitored with a rectal probe thermometer, and temperature is maintained at 37 °C by means of a heated surgical table. Blood samples for glucose analysis (10 il) are obtained from the tip of the tail every 15 minutes, and for lactate analysis (20 p.1) every... 

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